MBBS Microbiology Syllabus

MBBS Microbiology Syllabus

COURSE SYLLABUS FOR THEORY:-

Sl.No TOPICS TO BE COVERED TEACHING HOURS Domain
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MODULE

6

 

 

 

 

 

GENERAL MICROBIOLOGY 

1.      Introduction and Historical background

2.      Microscope

3.      Morphology of bacteria and Classification

4.      Physiology of bacteria including growth requirements & metabolism

5.      Sterilization and  Disinfection

6.      Culture media and culture methods

7.      Identification of bacteria

8.      Bacterial genetics and drug resistance to antimicrobial agents and methods of antimicrobial susceptibility testing

9.      Normal flora

10.  Microbial Pathogenicity

11.  Revision

PRACTICALS :

Hands on different types of microscopes, their principle, and handling and uses in Microbiology. Familiarize with various culture media, and characteristics of bacterial growth on various media and how to sterilize various media;

To demonstrate various simple biochemical tests used in identification of bacteria and perform simple tests like catalase and coagulase.

To demonstrate various types of equipment used in sterilization and show various disinfectants.

IMMUNOLOGY

1.      Introduction to immunology

2.      Antigen

3.      Antibodies

4.      Antigen-antibody reactions

5.      Immune response

6.      Complement System

7.      Hypersensitivity

8.      Autoimmunity, Immuno-deficiency

9.      Transplantation immunology & Major Histocompatibility  Complex

10.  Revision

 

PRACTICALS :

Familiarizewith various serological techniques like agglutination, ELISA. IFA. To demonstrate various types of equipment used in serology.

 

SYSTEMIC BACTERIOLOGY

1.      Staphylococcus

2.      Streptococcus and Pneumococcus

3.      Neisseria

4.      Hemophilus

5.      Corynebacterium

6.      Mycobacteria

7.      Mycobacterium Leprae

8.      Anaerobes (Clostridium  welchii, tetani, botulinum and other medically important clostridia

9.      Non sporing Anaerobes

10.  Enterobacteriaceae: E.coli, Klebsiella  and other medically important enteric bacteria

11.  Salmonella

12.  Shigella

13.  Vibrio

14.  Campylobacter & Helicobacter

15.  Pseudomonas

16.  Spirochetes (Treponema, Leptospira and Borrelia

17.  Actinomycosis& Nocardia

18.  Rickettsiae

19.  Chlamydia and Mycoplasma

20.  Legionella and Brucella

21.  Bacillus

22.  Bacteriology of  air, water, milk and food

23.  Revision

 

PRACTICALS :

Hands on different types of microscopes, their principle, and handling and uses in Microbiology. Familiarize with different specimens and sample collection methods for diagnosis of infectious diseases.

Familiarizewith  various culture media, and characteristics of bacterial growth on various media. To perform Gram staining, hanging drop coagulase, catalase and oxidase test. To make students carry out Albert’s stain. To demonstrate ZN stain, Culture characteristics and biochemical test for diagnosis and identification of   M.tuberculosis.

To make students carry out ZN stain. Familiarize various techniques of anaerobiosis, culture media and characteristics of bacterial growth on various media.

 

MYCOLOGY

1.      Introduction to mycology

2.      Superficial mycosis

3.      Subcutaneous mycosis

4.      Systemic mycosis

5.      Opportunistic fungal infections

6.      Revision

 

PRACTICALS :

Hands on preparation of KOH mount, various fungal culture medias and culture techniques for identification of fungal agents. Familiarize with different specimens and sample collection methods for diagnosis of fungal diseases.

To demonstrate the dermatophytes in wet mount preparation, true yeast and budding yeast cells in stained preparations and wet mount. To show them  the colonies of  Candida and Cryptococcus on Sabouraud’s Dextrose  agar

 

VIROLOGY

1.      General Virology

2.      Virus –Host interaction

3.      Laboratory diagnosis of viral infections

4.      Viral immunity

5.      Bacteriophage

6.      Adenoviruses, Pox viruses, Parvo viruses and other DNA viruses

7.      Herpes viruses

8.      Picornaviruses

9.      Rhabdoviruses

10.  Orthomyxoviruses

11.  Paramyxoviruses and other respiratory viruses

12.  Hepatitis viruses

13.  Arboviruses

14.  Rotavirus, Calciviruses, Astroviruses, and other gastro viruses

15.  Retroviruses

16.  Slow and Oncogenic Viruses

17.  Revision

PRACTICALS :

Familiarize with different specimens and sample collection methods for diagnosis of viral diseases. Familiarize with various serological techniques like agglutination, ELISA. IFA. HAI.

To demonstrate various types of equipment used in virology.

To Familiarize with egg inoculation and cell culture.

 

PARASITOLOGY

1.      Introduction to parasitology

2.      Amoebic protozoa: E. histolytica and others

3.      Free Living Amoebae

4.      Intestinal and genital flagellates

5.      Hemoflagellates: Leishmania

6.      Hemoflagellates: Trypanosomes

7.      Malaria parasite

8.      Coccidian parasites

9.      Cestodes: Taeniasaginata&solium

10.  Cestodes: Echinococcusgranulosus

11.  Trematodes : Schistosomiasis, Brief account of Fasciolahepatica and other trematodes

12.  Intestinal Nematodes

13.  Tissue Nematodes

14.  Revision

 

PRACTICALS :

Familiarize with different specimens and sample collection methods for diagnosis of parasitic diseases. To describe normal saline and iodine mount for stool specimens. To show them cysts and trophozoites of Entamoeba.

To show them mounted slides of amastigotes of Leishmania. To perform peripheral blood smear for diagnosis of blood parasites. To demonstrate some models and slides of filarial worms. Familiarize with various serological techniques like agglutination, ELISA. IFA. To demonstrate various types of equipment used in parasitology.

 

APPLIED MICROBIOLOGY

1.      Etiology and Lab Diagnosis of infectious diseases:

o   Sore Throat

o   Diarrheal disease

o   Meningitis

o   Urinary Tract Infections

o   Pyrexia of Unknown Origin

o   Sexual Transmitted Diseases

2.      Hospital acquired infections ( HAIs)

3.      Molecular Techniques

4.      Revision

 

PRACTICALS :

Familiarize with different specimens and sample collection methods for diagnosis of infectious diseases. To have clinical exercise on the above topics to show culture characters, biochemical and serological assays to diagnose the disease syndromes. Familiarize with various serological techniques like agglutination, ELISA. IFA.

To demonstrate various types of equipment used in diagnosis of infectious diseases.

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REFERENCEs::

Books recommended:

 

  1. Textbook of Medical Microbiology – Arora and Arora
  2. Textbook of Microbiology – R. Ananthanarayan
  3. K. JayaramPanikar
  4. Textbook of Medical Parasitology – Arora and Arora
  5. Textbook of Medical Parasitology – C. K. JayaramPanikar
  6. Textbook of Medical Parasitology – S.C.Parija
  7. Medical Mycology – Arora and Arora
  8. Practical Microbiology – Arora
  9. Practical Microbiology – Randhawa

Referencebooks:

  1. Mackie McCartneypractical Medical Microbiology- ColleeJG ,FraserAG
  2. Review ofMedical Microbiology(Lange) – Jawetz
  3. Immunology- Roitt Ivan M
  4. MedicalMicrobiology-DavidGreenwood,RichardStack,JohnPentherer
  5. Parasitology-KD Chatterjee
Admission 2017